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KMID : 0900919970210020139
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Korean journal of Animal Reproduction
1997 Volume.21 No. 2 p.139 ~ p.146
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Effect of Co-culture with Bovine and Porcine Oviductal Epithelial Cells on In Vitro Development of Mouse Embryos
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Abstract
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This experiment was carried out to evaluate the effect of mouse early embryonic development in vitro by co-culture with bovine and porcine oviductal epithelial cells(BOEC and POEC). The 2-cell embryos were collected from the oviducts of the superovulated and mated does with D-PBS/15% FCS at 48 hours after hCG injection. The in vitro developmental rate of blastocyst formation and the number of nuclei in the embryos were examined. For a comparative study of in vi패 and in vitro development, the fresh blastocyst which developed in vivo for 120 hours after hCG injection was collected from the uterus, and their numbers of nuclei were also counted. The higher developmental rates of blastocyst formation was a, pp.ared from 91% to 97% when the embryos were co-cultured with a monolayer of bovine or porcine oviductal epithelial cells in TCM 199 or Ham’s F-10 and MediCult IVF media. No significant difference in developmental rates was shown between bovine and porcine oviductal eptithelial cells. The number of nuclei in the embryos cultured for 72 hours under each conditions was significantly reduced it than blastocyst in vitro conditions. The number of nuclei in embryos cultured in TCM 199, Ham’s F-10 and Medicult IVF medium were counted 68.1pm6.00, 67.3pm4.49, 66.4pm5.64, and 94.3pm8.61, 92.5pm7.60, 92.1pm6.10 with BOEC and 93.3pm5.80, 92.9pm6.53, 92.3pm7.35 with POEC coculture, respectively. These numbers were lowered than 107.2pm7.43 in vivo conditions. In conclusions, the coculture between the mouse early embryos, and oviductal epithelial cells of BOEC and POEC give to improve the developmental and hatching rates of blastocyst but in vivo culture systems for the growth of nuclei were ineligible than in vitro conditions.
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KEYWORD
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Co-culture, Oviductal epithelial cells, Mouse embryo, Nuclei count
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